INSIG-1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-55244
![Western Blot: INSIG-1 AntibodyBSA Free [NB110-55244]](https://resources.bio-techne.com/images/products/INSIG-1-Antibody-Western-Blot-NB110-55244-img0002.jpg "Western Blot: INSIG-1 AntibodyBSA Free [NB110-55244]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Block/Neutralize, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.02 mg/ml
Product Specifications
Immunogen
A synthetic peptide representing an internal region of the human INSIG-1 protein (between residues 1-100) according to Swiss-Prot# O15503.
Localization
ER Membrane
Specificity
This is specific for Insig1 but not Insig2.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for INSIG-1 Antibody - BSA Free
Western Blot: INSIG-1 AntibodyBSA Free [NB110-55244]
Western Blot: INSIG-1 Antibody [NB110-55244] - Detection of Insig 1 in HepG2 lyaste using NB110-55244.![Immunocytochemistry/ Immunofluorescence: INSIG-1 Antibody - BSA Free [NB110-55244]](https://resources.bio-techne.com/images/products/INSIG-1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-55244-img0003.jpg "Immunocytochemistry/ Immunofluorescence: INSIG-1 Antibody - BSA Free [NB110-55244]")
Immunocytochemistry/ Immunofluorescence: INSIG-1 Antibody - BSA Free [NB110-55244]
Immunocytochemistry/Immunofluorescence: INSIG-1 Antibody [NB110-55244] - INSIG-1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Applications for INSIG-1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
2 ug/ml
Application Notes
This INSIG-1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band is seen at ~30 kDa representing the Insig1 protein. In ICC/IF positive staining was localized to the ER membrane in HepG2 cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 review rated 5 using NB110-55244 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS and 30% Glycerol
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1.02 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: INSIG-1
Alternate Names
CL6, CL-6, INSIG-1, INSIG-1 membrane protein, insulin induced gene 1, insulin-induced gene 1 protein, MGC1405
Gene Symbol
INSIG1
Additional INSIG-1 Products
Product Documents for INSIG-1 Antibody - BSA Free
Product Specific Notices for INSIG-1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...