INSIG-1 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-02969
Key Product Details
Species Reactivity
Human, Rat
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human INSIG-1 (NP_005533.2). LWWTFDRSRSGLGLGITIAFLATLITQFLVYNGVYQYTSPDFLYIRSWLPCIFFSGGVTVGNIGRQLAMGVPEKPHSD
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for INSIG-1 Antibody - Azide and BSA Free
![Western Blot: INSIG-1 AntibodyAzide and BSA Free [NBP3-02969]](https://resources.bio-techne.com/images/products/INSIG-1-Antibody-Western-Blot-NBP3-02969-img0001.jpg "Western Blot: INSIG-1 AntibodyAzide and BSA Free [NBP3-02969]")
Western Blot: INSIG-1 AntibodyAzide and BSA Free [NBP3-02969]
Western Blot: INSIG-1 Antibody [NBP3-02969] - Analysis of extracts of rat brain, using INSIG-1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL KitApplications for INSIG-1 Antibody - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: INSIG-1
Oxysterols regulate cholesterol homeostasis through liver X receptor (LXR) and sterol regulatory element-binding protein (SREBP) mediated signaling pathway. Insig1 binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Alternate Names
CL6, CL-6, INSIG-1, INSIG-1 membrane protein, insulin induced gene 1, insulin-induced gene 1 protein, MGC1405
Gene Symbol
INSIG1
Additional INSIG-1 Products
Product Documents for INSIG-1 Antibody - Azide and BSA Free
Product Specific Notices for INSIG-1 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov