Insulin Antibody (1F6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26195
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1F6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human Insulin [UniProt P01308]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Insulin Antibody (1F6)
Immunohistochemistry: Insulin Antibody (1F6) [NBP3-26195] -
Immunohistochemistry: Insulin Antibody (1F6) [NBP3-26195] - Image of Insulin Antibody (1F6) diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: Insulin Antibody (1F6) [NBP3-26195] -
Immunohistochemistry: Insulin Antibody (1F6) [NBP3-26195] - Image of Insulin Antibody (1F6) diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Applications for Insulin Antibody (1F6)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Insulin
Alternate Names
IDDM2, ILPR, INS, IRDN, MODY10
Gene Symbol
INS
Additional Insulin Products
Product Documents for Insulin Antibody (1F6)
Product Specific Notices for Insulin Antibody (1F6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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