Insulin Antibody (2D11-H5 (same as INS05)) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33146
![Immunohistochemistry-Paraffin: Insulin Antibody (2D11-H5 (same as INS05)) - Azide and BSA Free [NBP2-33146]](https://resources.bio-techne.com/images/products/Insulin-Antibody-2D11-H5-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33146-img0001.jpg "Immunohistochemistry-Paraffin: Insulin Antibody (2D11-H5 (same as INS05)) - Azide and BSA Free [NBP2-33146]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Porcine, Bovine
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2D11-H5 (same as INS05)
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Purified pig insulin, conjugated to KLH (Uniprot: P01308)
Localization
Cytoplasmic
Specificity
Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.
Marker
beta-Cell & Insulinoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-15195).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Insulin Antibody (2D11-H5 (same as INS05)) - Azide and BSA Free
Immunohistochemistry-Paraffin: Insulin Antibody (2D11-H5 (same as INS05)) - Azide and BSA Free [NBP2-33146]
Immunohistochemistry-Paraffin: Insulin Antibody (2D11-H5) - Azide and BSA Free [NBP2-33146] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin MonoclonalApplications for Insulin Antibody (2D11-H5 (same as INS05)) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 minutes at RT. No special pretreatment is required for staining of formalin/paraffin tissues.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Insulin
Alternate Names
IDDM2, ILPR, INS, IRDN, MODY10
Gene Symbol
INS
Additional Insulin Products
Product Documents for Insulin Antibody (2D11-H5 (same as INS05)) - Azide and BSA Free
Product Specific Notices for Insulin Antibody (2D11-H5 (same as INS05)) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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