Insulin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87485

Novus Biologicals, part of Bio-Techne
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NBP1-87485
NBP1-87485-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Insulin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYC

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Insulin Antibody

Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485]

Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485]

Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485] - Staining in human pancreas and liver tissues using anti-INS antibody. Corresponding INS RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485]

Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485]

Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485]

Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485]

Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485] - Staining of human pancreas shows high expression.

Applications for Insulin Antibody

Application
Recommended Usage

Flow Cytometry

Reported in scientific literature (PMID: 28824164)

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Insulin

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. (provided by RefSeq)

Alternate Names

IDDM2, ILPR, INS, IRDN, MODY10

Entrez Gene IDs

397415 (Porcine)

Gene Symbol

INS

Additional Insulin Products

Product Documents for Insulin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Insulin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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