Insulin R/CD220 Antibody (83-14) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81038
Recombinant Monoclonal Antibody
![Flow Cytometry: Insulin R/CD220 Antibody (83-14) - Chimeric - Azide and BSA Free [NBP2-81038]](https://resources.bio-techne.com/images/products/Insulin-R-CD220-Antibody-83-14-Flow-Cytometry-NBP2-81038-img0001.jpg "Flow Cytometry: Insulin R/CD220 Antibody (83-14) - Chimeric - Azide and BSA Free [NBP2-81038]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Porcine, Bovine, Sheep
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 83-14
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was raised by immunizing mice with IM-9 lymphocytes followed by purified insulin receptor.
Specificity
Recognises the extracellular domain of the Insulin R/CD220. Amino acids 469 - 592 (exon 7 and 8) of Insulin R/CD220.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for Insulin R/CD220 Antibody (83-14) - Chimeric - Azide and BSA Free
Flow Cytometry: Insulin R/CD220 Antibody (83-14) - Chimeric - Azide and BSA Free [NBP2-81038]
Flow Cytometry: Insulin R/CD220 Antibody (83-14) [NBP2-81038] - Flow-cytometry using the Insulin R/CD220 Antibody (83-14) [NBP2-81038]. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of 83-14 (NBP2-81038, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.Applications for Insulin R/CD220 Antibody (83-14) - Chimeric - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: Insulin R/CD220
Long Name
Insulin Receptor
Alternate Names
CD220, INSR, InsulinR
Gene Symbol
INSR
Additional Insulin R/CD220 Products
Product Documents for Insulin R/CD220 Antibody (83-14) - Chimeric - Azide and BSA Free
Product Specific Notices for Insulin R/CD220 Antibody (83-14) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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