Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # DATK32

Format

BSA Free

Concentration

0.5 mg/ml

View all Integrin alpha 4 beta 7/LPAM-1 Antibodies »

Product Summary for Integrin alpha 4 beta 7/LPAM-1 Antibody (DATK32) - BSA Free

Immunogen

The immunogen for this antibody was LPAM1.

Epitope

The monoclonal antibody DATK32 recognizes a conformational epitope on the heterodimer, integrin alpha 4 beta 7 (LPAM-1).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for Integrin alpha 4 beta 7/LPAM-1 Antibody (DATK32) - BSA Free

Flow Cytometry: Integrin alpha 4 beta 7/LPAM-1 Antibody (DATK32) - BSA Free [NBP1-43283]

Flow Cytometry: Integrin alpha 4 beta 7/LPAM-1 Antibody (DATK32) - BSA Free [NBP1-43283]

Flow Cytometry: Integrin alpha 4 beta 7/LPAM-1 Antibody (DATK32) [NBP1-43283] - Staining of C57BL/6 splenocytes with 0.5 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse Integrin alpha 4 beta 7 Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE . Cells in the lymphocyte gate were used for analysis.

Applications for Integrin alpha 4 beta 7/LPAM-1 Antibody (DATK32) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000
Application Notes
This DATK32 antibody has been tested tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Integrin alpha 4 beta 7/LPAM-1

The DATK32 monoclonal antibody binds to a combinatorial epitope of mouse LPAM-1 (alpha4beta7), which is a heterodimer of the the 154 kDa alpha4 subunit and the 130 kDa beta7 subunit. In mouse, the beta7 subunit is found on the majority of mature lymphocytes and a small population of thymocytes and bone marrow cells. LPAM-1 is an integrin involved in the transendothelial migration of lymphocytes across high endothelial venules (HEV), and is know to bind several ligands including MAdCAM-1, VCAM-1 and fibronectin. Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block LPAM-1-mediated cell adhesion.

Alternate Names

CD49d/beta7, LPAM-1, LPAM1

Entrez Gene IDs

3695 (Human); 3676 (Human); 16421 (Mouse); 16401 (Mouse)

Gene Symbol

ITGB7

UniProt

P13612, P26011, Q00651 (Mouse)

Additional Integrin alpha 4 beta 7/LPAM-1 Products

Product Documents for Integrin alpha 4 beta 7/LPAM-1 Antibody (DATK32) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Integrin alpha 4 beta 7/LPAM-1 Antibody (DATK32) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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