Integrin alpha 4/CD49d Antibody (HP2/1) [PE/Atto594]

Novus Biologicals, part of Bio-Techne | Catalog # NB120-22858PEATT594

Clone HP2/1 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB120-22858PEATT594
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Label

PE/Atto594 (Excitation = 488 nm, Emission = 627 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # HP2/1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Integrin alpha 4/CD49d Antibodies »

Product Specifications

Immunogen

JM leukaemia line

Reactivity Notes

Predicted cross-reactivities: Rhesus Monkey, Mustelid, Rat, Rabbit, Porcine, Llama, Mink, Equine, Goat, Feline, Cynomolgus monkey, Bovine

Specificity

Mouse anti Human CD49d monoclonal antibody, clone HP2/1 recognizes human CD49d also known as integrin alpha-4 or VLA-4 subunit alpha. CD49d is a ~150kDa single pass type 1 transmembrane glycoprotein with seven FG-GAP repeats, characteristic of alpha integrins, in its extracellular domain. CD49d can be proteolytically cleaved to yield framents of 80 and 70kDa (Hemler et al. 1987). CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form The Peyer patches-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue (Sackstein 2006) through its interaction with MadCam-1, preferentially expressed on Peyer's patch high endothelial venules and postcapillary venules in lamina propria (Briskin et al. 1997). Mouse anti human CD49d, clone HP2/1 binds to both intact and the 80kDa fragment of integrin alpha-4. CD49d is expressed on monocytes, T cells, B cells, thymocytes and Langerhans cells (de Graaf et al. 1995). Mouse anti Human CD49d, clone HP2/1 can be used in basic studies of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure and has been demonstrated to inhibit cell binding to soluble VCAM-1 (Weller et al. 1991).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Integrin alpha 4/CD49d Antibody (HP2/1) [PE/Atto594]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Integrin alpha 4/CD49d

VLA-4 is a cell surface heterodimer in the integrin superfamily of adhesion receptors. Anti-VLA-4 antibodies inhibited cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, whereas other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Alpha 4 stands apart from all other known integrin alpha subunit sequences because (i) alpha 4 has neither an inserted I-domain, nor a disulfide-linked C-terminal fragment, (ii) its sequence is the most unique and (iii) only alpha 4 has a potential protease cleavage site, near the middle of the coding region, which appears responsible for the characteristic 80,000 and 70,000 Mr fragments of alpha 4 (1). Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (2). The VLA-4 integrins are indispensable for embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (3).

Alternate Names

CD49d, ITGA4, VLA-4

Gene Symbol

ITGA4

Additional Integrin alpha 4/CD49d Products

Product Documents for Integrin alpha 4/CD49d Antibody (HP2/1) [PE/Atto594]

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Integrin alpha 4/CD49d Antibody (HP2/1) [PE/Atto594]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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