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Integrin alpha 4/CD49d Antibody (Natalizumab) [Alexa Fluor® 350]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75916AF350

Novus Biologicals, part of Bio-Techne
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NBP2-75916AF350

Key Product Details

Species Reactivity

Validated:

Human

Applications

Block/Neutralize, Flow Cytometry

Label

Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)

Antibody Source

Monoclonal Human IgG4 kappa Clone # Natalizumab

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Integrin alpha 4/CD49d Antibody (Natalizumab) [Alexa Fluor® 350]

Immunogen

The RAMOS cell line, a non-EBV releasing cell line, injected into mice.

Specificity

This antibody binds to the alpha 4 subunit of (alpha)4(beta)1 (also known as very late antigen 4 (VLA-4) or CD49d-CD29) and (alpha)4(beta)7 integrin

Clonality

Monoclonal

Host

Human

Isotype

IgG4 kappa

Scientific Data Images for Integrin alpha 4/CD49d Antibody (Natalizumab) [Alexa Fluor® 350]

Integrin alpha 4/CD49d Antibody (Natalizumab) [Alexa Fluor® 350]

Integrin alpha 4/CD49d Antibody (Natalizumab) [Alexa Fluor® 350] [NBP2-75916AF350] -

Integrin alpha 4/CD49d Antibody (Natalizumab) [Alexa Fluor® 350] [NBP2-75916AF350] - Vial of Alexa Fluor 350 conjugated antibody. Alexa Fluor 350 is optimally excited at 346 nm by the UV laser (350 or 355 nm) and has an emission maximum of 442 nm.

Applications for Integrin alpha 4/CD49d Antibody (Natalizumab) [Alexa Fluor® 350]

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Integrin alpha 4/CD49d

VLA-4 is a cell surface heterodimer in the integrin superfamily of adhesion receptors. Anti-VLA-4 antibodies inhibited cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, whereas other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Alpha 4 stands apart from all other known integrin alpha subunit sequences because (i) alpha 4 has neither an inserted I-domain, nor a disulfide-linked C-terminal fragment, (ii) its sequence is the most unique and (iii) only alpha 4 has a potential protease cleavage site, near the middle of the coding region, which appears responsible for the characteristic 80,000 and 70,000 Mr fragments of alpha 4 (1). Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (2). The VLA-4 integrins are indispensable for embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (3).

Alternate Names

CD49d, ITGA4, VLA-4

Gene Symbol

ITGA4

Additional Integrin alpha 4/CD49d Products

Product Documents for Integrin alpha 4/CD49d Antibody (Natalizumab) [Alexa Fluor® 350]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Integrin alpha 4/CD49d Antibody (Natalizumab) [Alexa Fluor® 350]



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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