Integrin alpha 4/CD49d Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77333

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Insect

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Integrin alpha 4/CD49d Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide from near the center of human Integrin alpha 4. The immunogen is located within amino acids 220 - 270 of Integrin alpha 4. Amino Acid Squence: TTNKYKAFLDKQNQV

Reactivity Notes

Insect (tick, Ixodes scapularis) reactivity reported from a verified customer review.

Specificity

At least two isoforms are known to exist. The immunogen to this antibody is within the Integrin alpha4 recombinant protein fragment and thus will recognize it.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Integrin alpha 4/CD49d Antibody - BSA Free

Western Blot: Integrin alpha 4/CD49d AntibodyBSA Free [NBP1-77333]

Western Blot: Integrin alpha 4/CD49d AntibodyBSA Free [NBP1-77333]

Western Blot: Integrin alpha 4/CD49d Antibody [NBP1-77333] - Western blot analysis of ISE6 (Tick, Ixodes scapularis, cell line) lysate. WB image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Integrin alpha 4/CD49d Antibody - BSA Free [NBP1-77333]

Immunocytochemistry/ Immunofluorescence: Integrin alpha 4/CD49d Antibody - BSA Free [NBP1-77333]

Immunocytochemistry/Immunofluorescence: Integrin alpha 4/CD49d Antibody [NBP1-77333] - Jurkat cells with Integrin alpha 4 antibody at 2 ug/mL.
Western Blot: Integrin alpha 4/CD49d AntibodyBSA Free [NBP1-77333]

Western Blot: Integrin alpha 4/CD49d AntibodyBSA Free [NBP1-77333]

Western Blot: Integrin alpha 4/CD49d Antibody [NBP1-77333] - Rat spleen tissue lysate with Integrin alpha 4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Applications for Integrin alpha 4/CD49d Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

2 ug/mL

Western Blot

1 ug/mL
Application Notes
Despite its predicted molecular weight, Integrin alpha 4 will often run at higher molecular weight in SDS-PAGE.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-77333 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Background: Integrin alpha 4/CD49d

The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells. Despite its predicted molecular weight, Integrin alpha4 will often run at higher molecular weight in SDS-PAGE. At least two isoforms are known to exist. The immunogen to this antibody is within the Integrin alpha4 recombinant protein fragment and thus will recognize it.

Alternate Names

CD49d, ITGA4, VLA-4

Entrez Gene IDs

3676 (Human); 16401 (Mouse)

Gene Symbol

ITGA4

UniProt

P13612

Additional Integrin alpha 4/CD49d Products

Product Documents for Integrin alpha 4/CD49d Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Integrin alpha 4/CD49d Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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