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Integrin alpha 5/CD49e Antibody (10F6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37666

Novus Biologicals, part of Bio-Techne
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NBP2-37666-0.025ml
NBP2-37666

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 10F6

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

Purified recombinant fragment of human Integrin alpha 5/CD49e expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Integrin alpha 5/CD49e Antibody (10F6) - BSA Free

Western Blot: Integrin alpha 5/CD49e Antibody (10F6)BSA Free [NBP2-37666]

Western Blot: Integrin alpha 5/CD49e Antibody (10F6)BSA Free [NBP2-37666]

Western Blot: Integrin alpha 5/CD49e Antibody (10F6) [NBP2-37666] - Western blot analysis using ITGA5 mouse mAb against ITGA5-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry: Integrin alpha 5/CD49e Antibody (10F6) - BSA Free [NBP2-37666]

Immunohistochemistry: Integrin alpha 5/CD49e Antibody (10F6) - BSA Free [NBP2-37666]

Immunohistochemistry: Integrin alpha 5/CD49e Antibody (10F6) [NBP2-37666] - Immunohistochemical analysis of paraffin-embedded human Placenta tissues using ITGA5 mouse mAb
Flow Cytometry: Integrin alpha 5/CD49e Antibody (10F6) - BSA Free [NBP2-37666]

Flow Cytometry: Integrin alpha 5/CD49e Antibody (10F6) - BSA Free [NBP2-37666]

Flow Cytometry: Integrin alpha 5/CD49e Antibody (10F6) [NBP2-37666] - Flow cytometric analysis of Hela cells using ITGA5 mouse mAb (green) and negative control (purple).

Applications for Integrin alpha 5/CD49e Antibody (10F6) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha 5/CD49e

CD49e is a type I integral membrane glycoprotein, known as alpha5 integrin, VLA-5 alpha chain. It forms noncovalent heterodimer with integrin beta1 (CD29). CD49e contains two disulfide-linked subunits of 135 kDa and 24 kDa, and is mainly expressed on thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, and some myeloid leukemia cells. CD49e/CD29 complex binds to fibronection and neural adhesion molecule L1. It mediates monocytes migration, involves in the regulation of cells survival and apoptosis, and co-stimulates T cell activation.

Alternate Names

CD49e, ITGA5, VLA-5 alpha

Gene Symbol

ITGA5

Additional Integrin alpha 5/CD49e Products

Product Documents for Integrin alpha 5/CD49e Antibody (10F6) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Integrin alpha 5/CD49e Antibody (10F6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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