Integrin alpha 5/CD49e Antibody (CL6951)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76520

Novus Biologicals, part of Bio-Techne
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NBP2-76520
NBP2-76520-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL6951

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Integrin alpha 5/CD49e Antibodies »

Product Summary for Integrin alpha 5/CD49e Antibody (CL6951)

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DQPQKEEDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTRVTGLNCTTNHPINPKGLELDPEGSLHHQQKREAPSRSSASSGPQILKCPEAECFRLRCELGPLHQQESQSLQLHFRVWA

Epitope

EEDLGPAVHHVYELI

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85)% and Rat (82)%

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Integrin alpha 5/CD49e Antibody (CL6951)

Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520]

Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520]

Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ITGA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520]

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520]

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520]

Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520]

Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Analysis in human cell line U-87 MG.
View 4 more images

Applications for Integrin alpha 5/CD49e Antibody (CL6951)

Application
Recommended Usage

Immunohistochemistry

1:10000 - 1:20000

Immunohistochemistry-Paraffin

1:10000 - 1:20000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, containing 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha 5/CD49e

CD49e is a type I integral membrane glycoprotein, known as alpha5 integrin, VLA-5 alpha chain. It forms noncovalent heterodimer with integrin beta1 (CD29). CD49e contains two disulfide-linked subunits of 135 kDa and 24 kDa, and is mainly expressed on thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, and some myeloid leukemia cells. CD49e/CD29 complex binds to fibronection and neural adhesion molecule L1. It mediates monocytes migration, involves in the regulation of cells survival and apoptosis, and co-stimulates T cell activation.

Alternate Names

CD49e, ITGA5, VLA-5 alpha

Gene Symbol

ITGA5

Additional Integrin alpha 5/CD49e Products

Product Documents for Integrin alpha 5/CD49e Antibody (CL6951)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Integrin alpha 5/CD49e Antibody (CL6951)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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