Integrin alpha 6/CD49f Antibody (GoH3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43400

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine

Cited:

Human, Bovine

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Cited:

Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # GoH3

Format

BSA Free

Concentration

1.0 mg/ml

View all Integrin alpha 6/CD49f Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD49f.

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 26254531)

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for Integrin alpha 6/CD49f Antibody (GoH3) - BSA Free

Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) - BSA Free [NBP1-43400]

Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) - BSA Free [NBP1-43400]

Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) [NBP1-43400] - Analysis using the FITC conjugate of NBP1-43400. Staining of Human peripheral blood lymphocytes with GoH3 FITC.
Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) - BSA Free [NBP1-43400]

Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) - BSA Free [NBP1-43400]

Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) [NBP1-43400] - Staining of normal human peripheral blood cells with 0.25 ug of Rat IgG2a Isotype Control Purified (NBP1-43400) (open histogram) or 0.25 ug of Anti-Human/Mouse CD49f (Integrin alpha 6) Purified (filled histogram) followed by Anti-Rat IgG PE. Cells in the lymphocyte gate were used for analysis.
Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) - BSA Free [NBP1-43400]

Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) - BSA Free [NBP1-43400]

Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) [NBP1-43400] - Analysis using the Biotin conjugate of NBP1-43400. Staining of normal human peripheral blood cells with 0.25 ug of Rat IgG2a k Isotype Control Biotin (black) or 0.125 ug of Anti-Human/Mouse CD49f Integrin a6 Biotin (purple) followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.

Applications for Integrin alpha 6/CD49f Antibody (GoH3) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
This GoH3 (GoH3) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Integrin alpha 6/CD49f

The GoH3 monoclonal antibody reacts with mouse and human CD49f, also known as integrin alpha 6, very late activation antigen 6 (VLA-6 alpha chain), and platelet gpIc. CD49f is a 120 kD transmembrane protein. CD49f associates with CD29, the integrin beta 1 chain, to form the VLA-6 complex CD49f also associates with CD104, the integrin beta 4 chain, to form the alpha 6 beta 4 complex. CD49f is expressed primarily on T cells, monocytes, platelets, epithelial and endothelial cells. CD49f expression has also been found on germinal center B cells. The GoH3 antibody is cross-reactive to integrin alpha 6 on human, mouse and bovine cells. This antibody has also been reported to have functional activity in blocking the binding of integrin alpha 6 to laminin.

Alternate Names

CD49f, ITGA6, Platelet gpl, VLA-6

Gene Symbol

ITGA6

Additional Integrin alpha 6/CD49f Products

Product Documents for Integrin alpha 6/CD49f Antibody (GoH3) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Integrin alpha 6/CD49f Antibody (GoH3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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