Integrin alpha E/CD103 Antibody (2E7) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28124

Novus Biologicals, part of Bio-Techne
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NBP1-28124
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NBP1-28126
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Armenian Hamster IgG2 Clone # 2E7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Integrin alpha E/CD103 Antibodies »

Product Specifications

Immunogen

C57BL/6J mouse intestinal intraepithelial lymphocytes (IEL)

Specificity

The CD103/alpha IELbeta7 integrin complex (Mr 160 and 115 kDa peptides).

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG2

Scientific Data Images for Integrin alpha E/CD103 Antibody (2E7) [FITC]

Flow Cytometry: Integrin alpha E/CD103 Antibody (2E7) [FITC] [NBP1-28124] - CD-1 mouse mesenteric lymph node cells were stained with Hamster Anti-Mouse CD103-FITC (NBP1-28124) and Rat Anti-Mouse CD3 Epsilon-PE.
Flow Cytometry: Integrin alpha E/CD103 Antibody (2E7) [FITC] [NBP1-28124] - BALB/c mesenteric lymph node cells were first labeled with Hamster Anti-Mouse CD103-BIOT, and then double-stained with streptavidin-FITC and Rat Anti-Mouse CD8a-R-PE, clone 53-6.7. Small lymphocytes were gated and analyzed on a Flow cytometer

Applications for Integrin alpha E/CD103 Antibody (2E7) [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Integrin alpha E/CD103

CD103 is a member of the integrin series of adhesion molecules. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and approx. 20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. (1-3) In non-epithelial CD103+ T cells there is a bias toward expression on CD8+.cells. The 2E7 mAb exhibits antigen immunoprecipitation patterns similar if not identical to the rat anti-mouse CD103 mAb M290. (1) The 2E7 mAb is reported to have signal-inducing activity in a redirected lysis assay and to co-stimulate IEL and CD8+ lymph node cells in conjunction with anti-TCR in vitro, which runs counter to the usual inhibition of CTL by anti-integrins. (1) This co-stimulatory activity is also shared by the mAb M290. (4) The property of costimulation is not unique to the mouse since the combination of anti-human alpha IEL plus anti-TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated. (5)

Alternate Names

CD103, HUMINAE, ITGAE

Gene Symbol

ITGAE

Additional Integrin alpha E/CD103 Products

Product Documents for Integrin alpha E/CD103 Antibody (2E7) [FITC]

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Integrin alpha E/CD103 Antibody (2E7) [FITC]

Protect conjugated forms from light.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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