Integrin alpha E/CD103 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98926

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Integrin alpha E/CD103 Antibodies »

Product Summary for Integrin alpha E/CD103 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Integrin alpha E/CD103.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Integrin alpha E/CD103 Antibody

Western Blot: Integrin alpha E/CD103 Antibody [NBP2-98926]

Western Blot: Integrin alpha E/CD103 Antibody [NBP2-98926]

Western Blot: Integrin alpha E/CD103 Antibody [NBP2-98926] - Anti-Integrin alpha E/CD103 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 130 kDa. Observed band size: 120 kDa (We are unsure as to the identity of these extra bands).
Immunocytochemistry/ Immunofluorescence: Integrin alpha E/CD103 Antibody [NBP2-98926]

Immunocytochemistry/ Immunofluorescence: Integrin alpha E/CD103 Antibody [NBP2-98926]

Immunocytochemistry/Immunofluorescence: Integrin alpha E/CD103 Antibody [NBP2-98926] - Immunofluorescence staining of Integrin alpha E/CD103 in HepG2 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human Integrin alpha E/CD103 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red), and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Western Blot: Integrin alpha E/CD103 Antibody [NBP2-98926]

Western Blot: Integrin alpha E/CD103 Antibody [NBP2-98926]

Western Blot: Integrin alpha E/CD103 Antibody [NBP2-98926] - Anti-Integrin alpha E/CD103 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 130 kDa. Observed band size: 120 kDa (We are unsure as to the identity of these extra bands).

Applications for Integrin alpha E/CD103 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha E/CD103

CD103 is a member of the integrin series of adhesion molecules. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and approx. 20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+.cells.

Alternate Names

CD103, HUMINAE, ITGAE

Gene Symbol

ITGAE

Additional Integrin alpha E/CD103 Products

Product Documents for Integrin alpha E/CD103 Antibody

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Certificate of Analysis

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Product Specific Notices for Integrin alpha E/CD103 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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