Integrin alpha L/CD11a Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51967

Novus Biologicals, part of Bio-Techne
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NBP1-51967
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Integrin alpha L/CD11a Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-YQVRIQPSIHDHN corresponding to internal region according to NP_002200.2, NP_001107852.1.

Epitope

C-YQVRIQPSIHDHN

Specificity

This antibody is expected to recognize both reported isoforms (NP_002200.2; NP_001107852.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Integrin alpha L/CD11a Antibody

Western Blot: Integrin alpha L/CD11a Antibody [NBP1-51967]

Western Blot: Integrin alpha L/CD11a Antibody [NBP1-51967]

Western Blot: Integrin alpha L/CD11a Antibody [NBP1-51967] - (0.5ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Integrin alpha L/CD11a Antibody [NBP1-51967]

Immunohistochemistry-Paraffin: Integrin alpha L/CD11a Antibody [NBP1-51967]

Immunohistochemistry-Paraffin: Integrin alpha L/CD11a Antibody [NBP1-51967] - (2ug/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry-Paraffin: Integrin alpha L/CD11a Antibody [NBP1-51967]

Immunohistochemistry-Paraffin: Integrin alpha L/CD11a Antibody [NBP1-51967]

Immunohistochemistry-Paraffin: Integrin alpha L/CD11a Antibody [NBP1-51967] - (3.8ug/ml) Staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Integrin alpha L/CD11a Antibody

Application
Recommended Usage

Immunohistochemistry

4 - 6 ug/ml

Immunohistochemistry-Paraffin

4-6 ug/ml

Peptide ELISA

1:16000

Western Blot

0.5-2 ug/ml
Application Notes
WB: Approx. 200 kDa band observed in human spleen and tonsil lysates (calculated MW of 129 kDa band according to NP_002200.2). The observed molecular weight corresponds to earlier findings with different antibodies from other sources. IHC-P: Human liver shows selected staining of cells within aggregated splenocytes of the white pulp.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Integrin alpha L/CD11a

CD11a is a 180 kD glycoprotein, also known as alpha-L integrin, LFA-1 alpha, Ly-15, or Ly-21. It is a member of the integrin family, primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes. In association with CD18, the CD11a/CD18 complex forms LFA-1. CD11a plays an important role in intercellular adhesion and costimulation by binding its ligands, ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50). The 2D7 antibody can block CD11a-mediated cellular adhesion.

Alternate Names

CD11a, ITGAL, LFA-1 alpha, Ly-15, Ly-21

Entrez Gene IDs

3683 (Human)

Gene Symbol

ITGAL

UniProt

NP_002200.2 (Human)

Additional Integrin alpha L/CD11a Products

Product Documents for Integrin alpha L/CD11a Antibody

Product Datasheets

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Product Specific Notices for Integrin alpha L/CD11a Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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