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Integrin alpha L/CD11a Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14902

Novus Biologicals, part of Bio-Techne
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NBP2-14902

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Primate (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Integrin alpha L/CD11a Antibody

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Integrin alpha L/CD11a. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (97%), Bovine (83%), Sheep (84%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

129 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Integrin alpha L/CD11a Antibody

Western Blot: Integrin alpha L/CD11a Antibody [NBP2-14902]

Western Blot: Integrin alpha L/CD11a Antibody [NBP2-14902]

Western Blot: Integrin alpha L/CD11a Antibody [NBP2-14902] - Sample (30 ug of whole cell lysate) A: THP-1 7.5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Integrin alpha L/CD11a Antibody [NBP2-14902]

Immunocytochemistry/ Immunofluorescence: Integrin alpha L/CD11a Antibody [NBP2-14902]

Immunocytochemistry/Immunofluorescence: Integrin alpha L/CD11a Antibody [NBP2-14902] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD11a protein stained by CD11a antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um

Applications for Integrin alpha L/CD11a Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Integrin alpha L/CD11a

ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CD11a, ITGAL, LFA-1 alpha, Ly-15, Ly-21

Gene Symbol

ITGAL

UniProt

Additional Integrin alpha L/CD11a Products

Product Documents for Integrin alpha L/CD11a Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Integrin alpha L/CD11a Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.