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Integrin alpha V/CD51 Antibody (4G7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23957

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-23957

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 4G7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Integrin alpha V/CD51 Antibody (4G7)

Immunogen

A synthesized peptide derived from human Integrin alpha V/CD51. UniProt ID (P06756).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Integrin alpha V/CD51 Antibody (4G7)

Integrin alpha V/CD51 Antibody (4G7)

Immunohistochemistry/Paraffin: Integrin alpha V/CD51 Antibody (4G7) [NBP3-23957] -

IHC image of Integrin alpha V antibody diluted at 1:100 and staining in paraffin-embedded human colon cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Integrin alpha V/CD51 Antibody (4G7)

Immunohistochemistry/Paraffin: Integrin alpha V/CD51 Antibody (4G7) [NBP3-23957] -

IHC image of Integrin alpha V antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for Integrin alpha V/CD51 Antibody (4G7)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

phosphate buffered saline, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Integrin alpha V/CD51

Integrin alpha V chain interacts with the integrin beta 3 subunit/CD61 to form the alpha-V-beta-3 heterodimer/vitronectin receptor. It is expressed on endothelial cells, some activated leukocytes, NK cells, macrophages, neutrophils, and platelets. Integrin alpha V also forms heterodimers with the integrin beta 1, beta 5, beta 6, and beta 8 subunits. Alpha-V-beta-3 is an activation dependent receptor for platelet attachment and spreading on vitronectin and other matrix components.

Alternate Names

CD51, ITGAV, MSK8, Vitronectin Receptor alpha, VNRA

Gene Symbol

ITGAV

Additional Integrin alpha V/CD51 Products

Product Documents for Integrin alpha V/CD51 Antibody (4G7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Integrin alpha V/CD51 Antibody (4G7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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