Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-383

Clone MEM-101A was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB500-383
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Key Product Details

Species Reactivity

Validated:

Human, Porcine, Canine, Mouse (Negative)

Cited:

Porcine, Canine

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-101A

Format

BSA Free

Concentration

1 mg/ml

View all Integrin beta 1/CD29 Antibodies »

Product Specifications

Immunogen

Raji Burkitt's lymphoma cell line (NM_002211.3).

Specificity

The antibody MEM-101A reacts with CD29 (integrin beta1 chain), a 130 kDa single chain type I glycoprotein expressed as a heterodimer (non-covalently associated with the integrin alpha subunits 1-6). CD29 is broadly expressed on majority of hematopoietic and non-hematopoietic cells (leukocytes, platelets, fibroblasts, endothelial cells, epithelial cells and mast cells). HLDA VI; WS Code AS A048

Marker

Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free

Western Blot: Integrin beta 1/CD29 Antibody (MEM-101A)BSA Free [NB500-383]

Western Blot: Integrin beta 1/CD29 Antibody (MEM-101A)BSA Free [NB500-383]

Western Blot: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-Integrin beta 1 Antibody (MEM-101A). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.
Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free [NB500-383]

Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free [NB500-383]

Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Staining of human peripheral blood with anti-human CD29 (MEM-101A) PE.
Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free [NB500-383]

Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free [NB500-383]

Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Surface staining of human peripheral blood with anti-human CD29 (MEM-101A) APC conjugated.

Applications for Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free

Application
Recommended Usage

Flow Cytometry

2 ug/ml

Immunoprecipitation

1:50

Western Blot

2 ug/ml
Application Notes
Purity is > 95% (by SDS-PAGE). Western Blot - Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS Application note: Non-reducing conditions. SDS-PAGE (6% separating gel, 4% stacking gel). Recommended secondary antibody - anti mouse IgG/HRP, dilution 1:2000, 60 min on vertical incubator. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Integrin beta 1/CD29

CD29 (beta1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CD49a-f) determining the binding properties of beta1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most beta1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of beta1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a beta1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta.

Alternate Names

CD29, ITGB1

Gene Symbol

ITGB1

Additional Integrin beta 1/CD29 Products

Product Documents for Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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