Integrin beta 1/CD29 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16974

Novus Biologicals, part of Bio-Techne
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NBP2-16974
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Canine

Cited:

Rat

Predicted:

Bovine (97%), Chicken (94%), Feline (98%), Porcine (98%), Rat (97%), Sheep (98%), Xenopus (91%). Backed by our 100% Guarantee.

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Chemotaxis, IF/IHC, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Integrin beta 1/CD29 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Integrin beta 1/CD29. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (91%), Cat (98%).

Localization

Cell membrane; Single-pass type I membrane protein; Melanosome

Marker

Stem Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Integrin beta 1/CD29 Antibody

Western Blot: Integrin beta 1/CD29 Antibody [NBP2-16974]

Western Blot: Integrin beta 1/CD29 Antibody [NBP2-16974]

Western Blot: Integrin beta 1/CD29 Antibody [NBP2-16974] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody [NBP2-16974]

Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody [NBP2-16974]

Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody [NBP2-16974] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Western Blot: Integrin beta 1/CD29 Antibody [NBP2-16974]

Western Blot: Integrin beta 1/CD29 Antibody [NBP2-16974]

Western Blot: Integrin beta 1/CD29 Antibody [NBP2-16974] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of human Integrin beta 1 / CD29-transfected 293T cells 7.5 % SDS-PAGEIntegrin beta 1 / CD29 antibody dilution: 1:5000
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Applications for Integrin beta 1/CD29 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Reported in scientific literature (PMID:33867854)

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Integrin beta 1/CD29

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CD29, ITGB1

Gene Symbol

ITGB1

UniProt

P05556

Additional Integrin beta 1/CD29 Products

Product Documents for Integrin beta 1/CD29 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Integrin beta 1/CD29 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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