Key Product Details

Species Reactivity

Human, Mouse, Porcine, Canine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2B1

Format

BSA Free

Concentration

1 mg/ml

View all Integrin beta 1D Antibodies »

Product Specifications

Immunogen

Derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Mouse immunized with a synthetic peptide corresponding to the C-terminal 24 amino acids of Integrin beta 1D including an appending N-terminal cysteine (CQENPIYKS-PINNFKNPNYGRKAGL) coupled to keyhole limpet hemocyanin.

Reactivity Notes

A broad species reactivity is expected based on the conserved nature of the epitope

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

This antibody recognizes specifically the cytoplasmic domain of Integrin beta 1D present in cardiac and skeletal muscle.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Scientific Data Images for Integrin beta 1D Antibody (2B1) - BSA Free

Immunocytochemistry/ Immunofluorescence: Integrin beta 1D Antibody (2B1) - BSA Free [NBP1-97734]

Immunocytochemistry/ Immunofluorescence: Integrin beta 1D Antibody (2B1) - BSA Free [NBP1-97734]

Immunocytochemistry/Immunofluorescence: Integrin beta 1D Antibody (2B1) [NBP1-97734] - Negative control: only secondary antibody applied.
Immunocytochemistry/ Immunofluorescence: Integrin beta 1D Antibody (2B1) - BSA Free [NBP1-97734]

Immunocytochemistry/ Immunofluorescence: Integrin beta 1D Antibody (2B1) - BSA Free [NBP1-97734]

Immunocytochemistry/Immunofluorescence: Integrin beta 1D Antibody (2B1) [NBP1-97734] - Frozen sections of porcine myocardium immunostained for integrin Beta 1D using a 1:200 dillution of 2B1

Applications for Integrin beta 1D Antibody (2B1) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:25-1:200

Western Blot

1:100-1:1000
Application Notes
This antibody is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications..
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS, pH 7.2

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin beta 1D

Alternate Names

CD29, FNRB, GPIIA, integrin beta-1, integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12), MDF2, MSK12, VLAB, VLA-BETA

Gene Symbol

ITGB1

Additional Integrin beta 1D Products

Product Documents for Integrin beta 1D Antibody (2B1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Integrin beta 1D Antibody (2B1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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