Integrin beta 2/CD18 Antibody (10E12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47440

Novus Biologicals, part of Bio-Techne
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NBP1-47440
NBP1-47440-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 10E12

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Integrin beta 2/CD18 Antibodies »

Product Summary for Integrin beta 2/CD18 Antibody (10E12) - BSA Free

Immunogen

Purified recombinant fragment of Integrin beta 2/CD18 expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Integrin beta 2/CD18 Antibody (10E12) - BSA Free

Immunocytochemistry/ Immunofluorescence: Integrin beta 2/CD18 Antibody (10E12) - BSA Free [NBP1-47440]

Immunocytochemistry/ Immunofluorescence: Integrin beta 2/CD18 Antibody (10E12) - BSA Free [NBP1-47440]

Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (10E12) [NBP1-47440] - Confocal immunofluorescence analysis of HL60 cells using anti-CD18 monoclonal antioby (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody (10E12) - BSA Free [NBP1-47440]

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody (10E12) - BSA Free [NBP1-47440]

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody (10E12) [NBP1-47440] - CD18 cells in mouse brain tumor tissue. Staining for antigen retrieval for 30 minutes. Primary antibody dilution is 1:200. Incubated overnight at 4 degree C. A DAB staining kit was used. IHC image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Integrin beta 2/CD18 Antibody (10E12) - BSA Free [NBP1-47440]

Immunocytochemistry/ Immunofluorescence: Integrin beta 2/CD18 Antibody (10E12) - BSA Free [NBP1-47440]

Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (10E12) [NBP1-47440] - Confocal immunofluorescence analysis of BCBL-1 cells (left) and L1210 cells (right) using anti-CD18 monoclonal antioby (red). Blue: DRAQ5 fluorescent DNA dye.

Applications for Integrin beta 2/CD18 Antibody (10E12) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000
Application Notes
This Integrin beta 2/CD18 Antibody (10E12) is validated for IHC from a verified customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-47440 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin beta 2/CD18

CD18, integrin b2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (b2) integrins: aLb2 (CD11a/CD18, LFA-1), aMb2 (CD11b/CD18, Mac-1, CR3), aXb2 (CD11c/CD18) and aDb2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.

Alternate Names

CD18, ITGB2, LCAMB, LFA-1, LFA-1 beta, MAC-1 beta, MF17, p150,95 beta

Gene Symbol

ITGB2

Additional Integrin beta 2/CD18 Products

Product Documents for Integrin beta 2/CD18 Antibody (10E12) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Integrin beta 2/CD18 Antibody (10E12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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