Integrin beta 2/CD18 Antibody (MEM-48) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-379

Novus Biologicals, part of Bio-Techne
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NB500-379
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Radioimmunoassay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-48

Format

BSA Free

Concentration

1 mg/ml

View all Integrin beta 2/CD18 Antibodies »

Product Summary for Integrin beta 2/CD18 Antibody (MEM-48) - BSA Free

Immunogen

Leukocytes of a patient suffering from a LGL-type leukemia.

Specificity

The antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Integrin beta 2/CD18 Antibody (MEM-48) - BSA Free

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody (MEM-48) - BSA Free [NB500-379]

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody (MEM-48) - BSA Free [NB500-379]

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody (MEM-48) [NB500-379] - Staining of human spleen (paraffin sections) using anti-CD18 (MEM-48).
Flow Cytometry: Integrin beta 2/CD18 Antibody (MEM-48) - BSA Free [NB500-379]

Flow Cytometry: Integrin beta 2/CD18 Antibody (MEM-48) - BSA Free [NB500-379]

Flow Cytometry: Integrin beta 2/CD18 Antibody (MEM-48) [NB500-379] - Human peripheral blood with anti-CD18 (MEM-48) APC.
Radioimmunoassay: Integrin beta 2/CD18 Antibody (MEM-48)BSA Free [NB500-379]

Radioimmunoassay: Integrin beta 2/CD18 Antibody (MEM-48)BSA Free [NB500-379]

Radioimmunoassay: Integrin beta 2/CD18 Antibody (MEM-48) [NB500-379] - Analysis (non-reducing conditions) of whole cell lysate using anti-human CD18 (MEM-48). Lane 1: KG-1a human acute myeloid leukemia cell line Lane 2: HPB-ALL human peripheral blood T cell leukemia cell line

Applications for Integrin beta 2/CD18 Antibody (MEM-48) - BSA Free

Application
Recommended Usage

Flow Cytometry

2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:200

Immunohistochemistry-Paraffin

10 ug/ml

Immunoprecipitation

1:50

Radioimmunoassay

1:100-1:2000

Western Blot

2 ug/ml
Application Notes
Western Blot - Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS Application note: Non-reducing conditions. SDS-PAGE (6% separating gel). This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Integrin beta 2/CD18

CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.

Alternate Names

CD18, ITGB2, LCAMB, LFA-1, LFA-1 beta, MAC-1 beta, MF17, p150,95 beta

Gene Symbol

ITGB2

Additional Integrin beta 2/CD18 Products

Product Documents for Integrin beta 2/CD18 Antibody (MEM-48) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Integrin beta 2/CD18 Antibody (MEM-48) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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