Integrin beta 2/CD18 Antibody (MEM-48) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NB500-380

Novus Biologicals, part of Bio-Techne
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NB500-380
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NB500-379
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-48

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Integrin beta 2/CD18 Antibodies »

Product Summary for Integrin beta 2/CD18 Antibody (MEM-48) [FITC]

Immunogen

Leukocytes of a patient suffering from a LGL-type leukemia.

Specificity

The antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Integrin beta 2/CD18 Antibody (MEM-48) [FITC]

Flow Cytometry: Integrin beta 2/CD18 Antibody (MEM-48) [FITC] [NB500-380] - Surface staining of human peripheral blood with anti-CD18 (MEM-48) FITC.

Applications for Integrin beta 2/CD18 Antibody (MEM-48) [FITC]

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 106 cells in a suspension.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

PBS and 0.2% (w/v) high grade BSA (protease free)

Preservative

0.9% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Integrin beta 2/CD18

CD18, integrin a2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (a2) integrins: aLa2 (CD11a/CD18, LFA-1), aMa2 (CD11b/CD18, Mac-1, CR3), aXa2 (CD11c/CD18) and aDa2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.

Alternate Names

CD18, ITGB2, LCAMB, LFA-1, LFA-1 beta, MAC-1 beta, MF17, p150,95 beta

Gene Symbol

ITGB2

Additional Integrin beta 2/CD18 Products

Product Documents for Integrin beta 2/CD18 Antibody (MEM-48) [FITC]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Integrin beta 2/CD18 Antibody (MEM-48) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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