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Integrin beta 3/CD61 [p Tyr785] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-81995

Novus Biologicals, part of Bio-Techne
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NB100-81995
NB100-81995-0.025ml

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Summary for Integrin beta 3/CD61 [p Tyr785] Antibody

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human Integrin beta 3/CD61 around the phosphorylation site of tyrosine 785 (I-T-YP-R-G).

Modification

p Tyr785

Specificity

Detects endogenous levels of Integrin beta 3/CD61 only when phosphorylated at tyrosine 785.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Integrin beta 3/CD61 [p Tyr785] Antibody

Western Blot: Integrin beta 3/CD61 [p Tyr785] Antibody [NB100-81995]

Western Blot: Integrin beta 3/CD61 [p Tyr785] Antibody [NB100-81995]

Western Blot: Integrin beta 3/CD61 [p Tyr785] Antibody [NB100-81995] - Analysis of extract from A431 cells untreated or treated with Insulin (40ug/ml, 15min), using Integrin beta3 (phospho-Tyr785) antibody (Lane 1 and 2).
Western Blot: Integrin beta 3/CD61 [p Tyr785] Antibody [NB100-81995]

Western Blot: Integrin beta 3/CD61 [p Tyr785] Antibody [NB100-81995]

Western Blot: Integrin beta 3/CD61 [p Tyr785] Antibody [NB100-81995] - Analysis of extracts from HUVEC cells using Integrin b3(Phospho-Tyr785) Antibody and the same antibody preincubated with blocking peptide.

Applications for Integrin beta 3/CD61 [p Tyr785] Antibody

Application
Recommended Usage

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin beta 3/CD61

Human Integrin beta 3 (CD61) antigen is expressed on platelets, megakaryocytes, macrophages, osteoclasts and synovial lining cells. The integrin beta 3 chain of the vitronectin receptor and GPIIb/IIIa complex. The beta 3 integrin chain is a 90-110 kDa glycoprotein polypeptide which is expressed on platelets, megakaryocytes, macrophages, osteoclasts and synovial lining cells.

Alternate Names

CD61, GP3A, GPIIIA, INGRB3, ITGB3

Entrez Gene IDs

3690 (Human); 16416 (Mouse); 29302 (Rat)

Gene Symbol

ITGB3

UniProt

Additional Integrin beta 3/CD61 Products

Product Documents for Integrin beta 3/CD61 [p Tyr785] Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Integrin beta 3/CD61 [p Tyr785] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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