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Integrin beta 4/CD104 Antibody (439-9B) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43599

Clone 439-9B was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP1-43599-0.1mg
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Key Product Details

Species Reactivity

Human, Bovine

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Biotin

Antibody Source

Monoclonal Rat IgG2B Clone # 439-9B

Concentration

0.5 mg/ml

Product Specifications

Immunogen

The immunogen for this antibody was CD104.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Integrin beta 4/CD104 Antibody (439-9B) [Biotin]

Flow Cytometry: Integrin beta 4/CD104 Antibody (439-9B) [Biotin] [NBP1-43599] - Staining of the A549 cell line with 0.125 ug of Rat IgG2b k Isotype Control Biotin (open histogram) or 0.125 ug of Anti-Human CD104 (Integrin BETA 4) Biotin (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.

Applications for Integrin beta 4/CD104 Antibody (439-9B) [Biotin]

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500
Application Notes
For WB, use under non-reducing conditions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2) with 0.1% gelatin

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Integrin beta 4/CD104

The monoclonal antibody 439-9B recognizes human CD104, also known as integrin beta4. CD104 is a 202 kDa subunit that associates with integrin alpha6 (CD49f), thereby forming the laminin receptor. Multiple splice variants have been identified. Expression is found on epithelial cells and tumor cells and has been shown to be involved in cell-matrix and cell-cell interactions. The role of CD104 in tumorigenicity is well established. The monoclonal antibody 439-9B has been reported to have no effect on adhesion of human colon adenocarcinoma cells. Moreover, this antibody crossreacts to bovine.

Alternate Names

CD104, ITGB4

Gene Symbol

ITGB4

Additional Integrin beta 4/CD104 Products

Product Documents for Integrin beta 4/CD104 Antibody (439-9B) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Integrin beta 4/CD104 Antibody (439-9B) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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