Integrin beta 4/CD104 Antibody (450-9D)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-65599
![Immunohistochemistry-Frozen: Integrin beta 4/CD104 Antibody (450-9D) - BSA Free [NB100-65599]](https://resources.bio-techne.com/images/products/Integrin-beta-4-CD104-Antibody-450-9D-Immunohistochemistry-Frozen-NB100-65599-img0002.jpg "Immunohistochemistry-Frozen: Integrin beta 4/CD104 Antibody (450-9D) - BSA Free [NB100-65599]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 450-9D
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Purified alpha6 beta4 integrin from A431 cells
Reactivity Notes
Predicted cross-reactivities: Ferret, Mink, Mustelid
Specificity
NB100-65599 recognizes the human beta4 integrin (also known as CD104), a 205kD glycoprotein which associates with the alpha6 integrin to form the alpha6/beta4 complex. CD104 is expressed on epithelial cells, Schwann cells and various tumour cell lines. NB100-65599 recognizes an extracellular epitope on the CD104 molecule.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Integrin beta 4/CD104 Antibody (450-9D)
Immunohistochemistry-Frozen: Integrin beta 4/CD104 Antibody (450-9D) - BSA Free [NB100-65599]
Immunohistochemistry-Frozen: Integrin beta 4/CD104 Antibody (450-9D) [NB100-65599] - Staining of human tonsil cryostat section with Mouse anti human CD104Applications for Integrin beta 4/CD104 Antibody (450-9D)
Application
Recommended Usage
Flow Cytometry
1:25-1:100
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:500-1:1000
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.
Reviewed Applications
Read 1 review rated 4 using NB100-65599 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Integrin beta 4/CD104
Additional Integrin beta 4/CD104 Products
Product Documents for Integrin beta 4/CD104 Antibody (450-9D)
Product Specific Notices for Integrin beta 4/CD104 Antibody (450-9D)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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