Integrin beta 4/CD104 Antibody (UM-A9) [PE/Cy5.5]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34507PECY55
Clone UM-A9 was used by HLDA to establish CD designation.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
PE/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # UM-A9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Human squamous cell carcinoma (UM-SCC1)
Localization
Cell surface
Specificity
Recognizes a protein of 205kDa, which is identified as integrin beta-4 (ITGB4). Its epitope is localized in the extracellular domain of ITGB4 protein. Integrins are heterodimers comprised of alpha and beta subunits, that are non-covalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta-4 subunit, a receptor for the laminins. This subunit tends to associate with alpha-6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Applications for Integrin beta 4/CD104 Antibody (UM-A9) [PE/Cy5.5]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Integrin beta 4/CD104
Alternate Names
CD104, ITGB4
Gene Symbol
ITGB4
Additional Integrin beta 4/CD104 Products
Product Documents for Integrin beta 4/CD104 Antibody (UM-A9) [PE/Cy5.5]
Product Specific Notices for Integrin beta 4/CD104 Antibody (UM-A9) [PE/Cy5.5]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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