Involucrin Antibody (rIVRN/9323)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23830
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG2b Kappa Clone # rIVRN/9323
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human full-length Involucrin protein (IVRN)
Localization
Cytoplasm.
Specificity
It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this monoclonal antibody reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin.
Marker
Squamous Cell Terminal Differentiation Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Store at 2 to 8C. Non-hazardous. No MSDS required.
Applications for Involucrin Antibody (rIVRN/9323)
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.1-0.2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37C. Note than enzyme digestion is better than citrate buffer based epitope unmasking. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Involucrin
Alternate Names
involucrin
Gene Symbol
IVL
Additional Involucrin Products
Product Documents for Involucrin Antibody (rIVRN/9323)
Product Specific Notices for Involucrin Antibody (rIVRN/9323)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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