Involucrin Antibody (rIVRN/9323) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24321
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # rIVRN/9323
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human full-length Involucrin protein (IVRN)
Localization
Cytoplasm.
Specificity
It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this monoclonal antibody reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin.
Marker
Squamous Cell Terminal Differentiation Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Antibody without azide - store at -20 to -80C.
Applications for Involucrin Antibody (rIVRN/9323) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.1-0.2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37C. Note than enzyme digestion is better than citrate buffer based epitope unmasking. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Involucrin
Alternate Names
involucrin
Gene Symbol
IVL
Additional Involucrin Products
Product Documents for Involucrin Antibody (rIVRN/9323) - Azide and BSA Free
Product Specific Notices for Involucrin Antibody (rIVRN/9323) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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