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IQGAP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51980

Novus Biologicals, part of Bio-Techne
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NBP1-51980

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-KRQSGQTDPLQKEE corresponding to internal region according to NP_003861.1.

Epitope

C-KRQSGQTDPLQKEE

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for IQGAP1 Antibody

Western Blot: IQGAP1 Antibody [NBP1-51980]

Western Blot: IQGAP1 Antibody [NBP1-51980]

Western Blot: IQGAP1 Antibody [NBP1-51980] - (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for IQGAP1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 200 kDa band observed in human kidney and mouse kidney lysates (calculated MW of 189 kDa band according to NP_003861.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IQGAP1

This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.

Long Name

IQ Motif Containing GTPase Activating Protein 1

Alternate Names

p195, SAR1

Entrez Gene IDs

8826 (Human); 29875 (Mouse); 361598 (Rat)

Gene Symbol

IQGAP1

Additional IQGAP1 Products

Product Documents for IQGAP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IQGAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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