IQGAP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83143

Novus Biologicals, part of Bio-Techne
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NBP1-83143
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IQGAP1 Antibodies »

Product Summary for IQGAP1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NRALESGDVNTVWKQLSSSVTGLTNIEEENCQRYLDELMKLKAQAHAENNEFITWNDIQACVDHVNLVVQEEHERILAIGLINEALDEGDAQKTLQALQIPAAKLEGVLAEVAQHYQDTLIRAKREKAQEIQDESAVLWLDEIQGG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IQGAP1 Antibody

Western Blot: IQGAP1 Antibody [NBP1-83143]

Western Blot: IQGAP1 Antibody [NBP1-83143]

Western Blot: IQGAP1 Antibody [NBP1-83143] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: IQGAP1 Antibody [NBP1-83143]

Immunohistochemistry-Paraffin: IQGAP1 Antibody [NBP1-83143]

Immunohistochemistry-Paraffin: IQGAP1 Antibody [NBP1-83143] - Staining in human endometrium and skeletal muscle tissues using anti-IQGAP1 antibody. Corresponding IQGAP1 RNA-seq data are presented for the same tissues.
Western Blot: IQGAP1 Antibody [NBP1-83143]

Western Blot: IQGAP1 Antibody [NBP1-83143]

Western Blot: IQGAP1 Antibody [NBP1-83143] - Analysis in human cell line A-431.
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Applications for IQGAP1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IQGAP1

IQGAP1 encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.

Long Name

IQ Motif Containing GTPase Activating Protein 1

Alternate Names

p195, SAR1

Gene Symbol

IQGAP1

Additional IQGAP1 Products

Product Documents for IQGAP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IQGAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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