IRAK3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-96912

Novus Biologicals, part of Bio-Techne
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NB100-96912
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Mouse, Rat

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all IRAK3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-GHSYGSKPMEKR corresponding to internal region (near C-Terminus) according to NP_082955.2.

Epitope

GHSYGSKPMEKR

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for IRAK3 Antibody

Western Blot: IRAK3 Antibody [NB100-96912]

Western Blot: IRAK3 Antibody [NB100-96912]

Western Blot: IRAK3 Antibody [NB100-96912] - Staining (0.1ug/ml) of Rat Liver lysate (35ug protein in RIPA buffer). Detected with chemiluminescence.
IRAK3 Antibody

Western Blot: IRAK3 Antibody [NB100-96912] -

Western Blot: IRAK3 Antibody [NB100-96912] - (0.5ug/ml) staining of Mouse Bone Marrow (wildtype first lane, KO second lane) lysates (35ug protein in RIPA buffer). Primary incubation was overnight at 4C Detected with chemiluminescence. Data obtained from anonymous customer.

Applications for IRAK3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.03 - 0.1 ug/ml
Application Notes
WB: Approx 65-70 kDa band observed in Rat Liver lysates, and in preliminary testing of Rat Adrenal Gland lysate (calculated MW of 69.0 kDa according to NP_001101571.1 and 67.8 kDa according to NP_082955.2). An approx. 70 kDa band is observed in mouse bone marrow lysates but this band disappears in lysates from the knock-out mouse.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IRAK3

reduced expression of IRAK-1 and increased expression of IRAK-M after CpG DNA pretreatment resulted in the hyporesponsiveness of macrophages that leads to the protection of mice from hepatic injury and death caused by CpG DNA/D-GalN. Endotoxin tolerance was significantly reduced in macrophages from IRAK-M knockout mice. IRAK-M regulates TLR signaling and innate immune homeostasis. induction of IRAK-M and inhibition of kinase activity of IRAK-1 are crucial to PGN-induced tolerance in macrophages. IRAK-M is a key regulator of the bone loss that is due to osteoclastic resorption of bone. IRAK-M induction negatively regulates Toll-like receptor-dependent interleukin-12 p40 production in macrophages in the presence of Mycobacterium tuberculosis lipoarabinomannan. These findings indicate that IRAK-M selectively attenuates p38 activation and inhibits innate immunity through stabilizing MKP-1. IRAK-M is directly involved in the regulation of both innate and adaptive immune signaling processes, and deletion of IRAK-M enhances host anti-tumor immune response.

Long Name

Interleukin-1 Receptor-Associated Kinase 3

Alternate Names

ASRT5, IRAK-M

Entrez Gene IDs

73914 (Mouse); 314870 (Rat)

Gene Symbol

IRAK3

UniProt

NP_082955.2 (Mouse)

Additional IRAK3 Products

Product Documents for IRAK3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IRAK3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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