IRAK3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83094

Novus Biologicals, part of Bio-Techne
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NBP1-83094
NBP1-83094-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IRAK3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SWLDVRHIEKYVDQGKSGTRELLWSWAQKNKTIGDLLQVLQEMGHRRAIHLITNYGAVLSPSEKSYQEGGFPNILFKETANVTVDNVLIPEHNEKGVLLKSSISFQNIIEGTRNFHKDFLIGE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRAK3 Antibody

Western Blot: IRAK3 Antibody [NBP1-83094]

Western Blot: IRAK3 Antibody [NBP1-83094]

Western Blot: IRAK3 Antibody [NBP1-83094] - Analysis in control (vector only transfected HEK293T lysate) and IRAK3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: IRAK3 Antibody [NBP1-83094]

Immunocytochemistry/ Immunofluorescence: IRAK3 Antibody [NBP1-83094]

Immunocytochemistry/Immunofluorescence: IRAK3 Antibody [NBP1-83094] - Immunofluorescent staining of human cell line SiHa shows localization to vesicles.
Immunohistochemistry-Paraffin: IRAK3 Antibody [NBP1-83094]

Immunohistochemistry-Paraffin: IRAK3 Antibody [NBP1-83094]

Immunohistochemistry-Paraffin: IRAK3 Antibody [NBP1-83094] - Staining of human skeletal muscle shows low positivity in myocytes as expected.
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Applications for IRAK3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRAK3

IRAK3 is a novel member in the IRAK/Pelle family. IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and play a central role in IL-1R/TLR mediated inflammatory responses. Interleukin-1 (IL-1) and lipopolysaccharide (LPS)induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor associated kinase (IRAK and IRAK2) mediates the activation of NF-kB by IL-1/Toll receptors (Wesche H et al.1999,Muzio M et al.1997), which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in theIRAK/Pelle family was recently identified and designated IRAK-M (Cao Z et al.1996). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and thedominant negative mutants of IRAKs inhibit IL-1 orLPS induced NF-kB activation. Members inIRAK/Pelle family play a central role in IL-1R/TLRmediated inflammatory responses to cytokine IL-1and LPS.

Long Name

Interleukin-1 Receptor-Associated Kinase 3

Alternate Names

ASRT5, IRAK-M

Gene Symbol

IRAK3

Additional IRAK3 Products

Product Documents for IRAK3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IRAK3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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