IRAK4 Antibody (10H4)

Western Blot: IRAK4 Antibody (10H4) [NBP3-15153]

Western Blot: IRAK4 Antibody (10H4) [NBP3-15153] - Positive WB detected in: THP-1 whole cell lysate, U251 whole cell lysate. All lanes: IRAK4 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 52, 38 KDa. Observed band size: 55 KDa.

Flow Cytometry: IRAK4 Antibody (10H4) [NBP3-15153]

Flow Cytometry: IRAK4 Antibody (10H4) [NBP3-15153] - Overlay histogram showing Jurkat cells stained with IRAK4 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.