IRAK4 Antibody (2H9) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37575
Key Product Details
Species Reactivity
Human, Mouse, Monkey
Applications
Validated:
ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2H9
Format
BSA Free
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Purified recombinant fragment of human IRAK4 expressed in E. Coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IRAK4 Antibody (2H9) - BSA Free
Western Blot: IRAK4 Antibody (2H9)BSA Free [NBP2-37575]
Western Blot: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis using IRAK4 mouse mAb against THP-1 (1), Hela (2), K562 (3), MCF-7 (4), RAW264.7 (5), Jurkat (6) and Cos7 (7) cell lysate.Immunohistochemistry-Paraffin: IRAK4 Antibody (2H9) - BSA Free [NBP2-37575]
Immunohistochemistry-Paraffin: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis of human kidney cancer tissues using IRAK4 mouse mAb with DAB staining.Flow Cytometry: IRAK4 Antibody (2H9) - BSA Free [NBP2-37575]
Flow Cytometry: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis of Hela cells using IRAK4 mouse mAb (blue) and negative control (red).Applications for IRAK4 Antibody (2H9) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200 - 1:400
Immunohistochemistry
1:200 - 1:1000
Western Blot
1:500 - 1:2000
Application Notes
This IRAK4 Antibody (2H9) is validated for IP from a verified customer review.
Reviewed Applications
Read 1 review rated 5 using NBP2-37575 in the following applications:
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Format
BSA Free
Preservative
0.03% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IRAK4
Long Name
Interleukin-1 Receptor-Associated Kinase 4
Alternate Names
REN64
Gene Symbol
IRAK4
Additional IRAK4 Products
Product Documents for IRAK4 Antibody (2H9) - BSA Free
Product Specific Notices for IRAK4 Antibody (2H9) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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