IRAK4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-597

Novus Biologicals, part of Bio-Techne
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NB500-597-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Flow Cytometry, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all IRAK4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a mixture of synthetic peptides containing amino acids 38-54 and 120-136 of mouse IRAK-4.

Reactivity Notes

Rat reactivity reported in multiple pieces of scientific literature

Localization

Cell Membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRAK4 Antibody - BSA Free

Western Blot: IRAK4 Antibody [NB500-597]

Western Blot: IRAK4 Antibody [NB500-597]

Western Blot: IRAK4 Antibody [NB500-597] - Analysis of IRAK-4 in 30 ugs of NIH 3T3 cell lysate using this antibody at 1:500 dilution.

Applications for IRAK4 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:1000 - 1:2000
Application Notes
Immunoprecipitation: see Pacquelet et al (2007) for details.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRAK4

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). IL-1 receptor-associated kinases (IRAKs) are important mediators in the signal transduction of Toll/IL-1 receptor (TIR) family members (2). The cytoplasmic domains of TIR proteins interact with the adapter protein, MyD88. MyD88 then recruits IRAKs (IRAK-1, -2, and M), which in turn interact with other adapter molecules, such as TRAF6 to activate NF-kB and MAPK pathways. Recently, a new member of this family, IRAK-4 has been identified (3). IRAK-4 may act as an upstream activator of IRAK-1. IRAK-4 is important for LPS activation of TLRS. Mice lacking IRAK-4 are resistant to lethal doses of LPS and are also severely impaired in their responses to viral and bacterial challenges (4, 5).

Long Name

Interleukin-1 Receptor-Associated Kinase 4

Alternate Names

REN64

Entrez Gene IDs

51135 (Human); 266632 (Mouse)

Gene Symbol

IRAK4

UniProt

NP_001107654 (Human)

Additional IRAK4 Products

Product Documents for IRAK4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IRAK4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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