IRAK4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB500-597

Novus Biologicals, part of Bio-Techne
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NB500-597-0.1mg
NB500-597-0.05mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Flow Cytometry, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all IRAK4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a mixture of synthetic peptides containing amino acids 38-54 and 120-136 of mouse IRAK-4.

Reactivity Notes

Rat reactivity reported in multiple pieces of scientific literature

Localization

Cell Membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRAK4 Antibody

Western Blot: IRAK4 Antibody [NB500-597]

Western Blot: IRAK4 Antibody [NB500-597]

Western Blot: IRAK4 Antibody [NB500-597] - Analysis of IRAK-4 in 30 ugs of NIH 3T3 cell lysate using this antibody at 1:500 dilution.

Applications for IRAK4 Antibody

Application
Recommended Usage

Western Blot

1:1000 - 1:2000
Application Notes
Immunoprecipitation: see Pacquelet et al (2007) for details.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRAK4

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). IL-1 receptor-associated kinases (IRAKs) are important mediators in the signal transduction of Toll/IL-1 receptor (TIR) family members (2). The cytoplasmic domains of TIR proteins interact with the adapter protein, MyD88. MyD88 then recruits IRAKs (IRAK-1, -2, and M), which in turn interact with other adapter molecules, such as TRAF6 to activate NF-kB and MAPK pathways. Recently, a new member of this family, IRAK-4 has been identified (3). IRAK-4 may act as an upstream activator of IRAK-1. IRAK-4 is important for LPS activation of TLRS. Mice lacking IRAK-4 are resistant to lethal doses of LPS and are also severely impaired in their responses to viral and bacterial challenges (4, 5).

Long Name

Interleukin-1 Receptor-Associated Kinase 4

Alternate Names

REN64

Entrez Gene IDs

51135 (Human); 266632 (Mouse)

Gene Symbol

IRAK4

UniProt

NP_001107654 (Human)

Additional IRAK4 Products

Product Documents for IRAK4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IRAK4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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