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IRF3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76597

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Summary for IRF3 Antibody - BSA Free

Immunogen

Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human IRF3. The immunogen is located within amino acids 330 - 380 of IRF3. Amino Acid Squence: GESWPQDQPWTKRL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%).

Specificity

At least three isoforms of IRF3 are known to exist.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IRF3 Antibody - BSA Free

Western Blot: IRF3 AntibodyBSA Free [NBP1-76597]

Western Blot: IRF3 AntibodyBSA Free [NBP1-76597]

Western Blot: IRF3 Antibody [NBP1-76597] - Ramos whole cell lysate with IRF3 antibody at (A) 1, (B) 2, and (C) 4 ug/ml.
Immunocytochemistry/ Immunofluorescence: IRF3 Antibody - BSA Free [NBP1-76597]

Immunocytochemistry/ Immunofluorescence: IRF3 Antibody - BSA Free [NBP1-76597]

Immunocytochemistry/Immunofluorescence: IRF3 Antibody [NBP1-76597] - Mouse Kidney cells.
Immunohistochemistry-Paraffin: IRF3 Antibody - BSA Free [NBP1-76597]

Immunohistochemistry-Paraffin: IRF3 Antibody - BSA Free [NBP1-76597]

Immunohistochemistry-Paraffin: IRF3 Antibody [NBP1-76597] - Mouse kidney using IRF3 antibody at 2 ug/ml.

Applications for IRF3 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

1-4 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRF3

Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF3 is present in an inactive form in the cytoplasm of most cells. Following viral infection, IRF3 can be activated by IkappaB kinase-epsilon and TANK-binding kinase 1 (TBK1), whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA. IRF3 exists as at least two distinct isoforms.

Long Name

Interferon Regulatory Factor 3

Alternate Names

interferon regulatory factor 3, IRF-3

Gene Symbol

IRF3

Additional IRF3 Products

Product Documents for IRF3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IRF3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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