Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all IRF3 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 375 and 419 of mouse Interferon Regulatory Factor 3 using the numbering given in entry NP_058545.1 (GeneID 54131).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IRF3 Antibody

Western Blot: IRF3 Antibody [NBP1-78769]

Western Blot: IRF3 Antibody [NBP1-78769]

Western Blot: IRF3 Antibody [NBP1-78769] - Whole cell lysate from mouse bone marrow dendritic (D; 50 and 15 mcg for WB; 1 mg for IP, 20% of IP loaded), and NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-IRF3 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IRF3 was also immunoprecipitated by rabbit anti-IRF3 antibody NBP1-78768 which recognizes a disparate epitope.

Applications for IRF3 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:1000-1:2500
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IRF3

IRF3 is a family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) but not to the IRF-1 binding site PRD-I. Overexpression of IRF-3 stimulates expression of the IFN-stimulated gene 15 (ISG15) promoter, an ISRE-containing promoter. he high amino acid homology between IRF-3 and ISG factor 3 gamma polypeptide (ISGF3 gamma) and their similar binding properties indicate that, like ISGF3 gamma, IRF-3 may activate transcription by complex formation with other transcriptional factors, possibly members of the Stat family (1). IRF-3 is expressed constitutively in a variety of tissues, and the relative levels of IRF-3 mRNA do not change in virus-infected or IFN-treated cells. (2). In uninfected cells, the IRF-3 component of DRAF1 resides in the cytoplasm. The cytoplasmic localization of IRF-3 is dependent on a nuclear export signal, and IRF-3 is recognized by the chromosome region maintenance 1 (CRM1) (also known as exportin 1) shuttling receptor. Following infection and specific phosphorylation, IRF-3 accumulates in the nucleus where it associates with CBP and p300 (3).

Long Name

Interferon Regulatory Factor 3

Alternate Names

interferon regulatory factor 3, IRF-3

Entrez Gene IDs

54131 (Mouse)

Gene Symbol

IRF3

UniProt

P70671

Additional IRF3 Products

Product Documents for IRF3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IRF3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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