IRF5 Antibody (10T1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-04307

Novus Biologicals, part of Bio-Techne
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NBP1-04307
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 10T1

Format

BSA Free

Concentration

1.0 mg/ml

View all IRF5 Antibodies »

Product Specifications

Immunogen

Recombinant human IRF5 (176-240 aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for IRF5 Antibody (10T1) - BSA Free

Western Blot: IRF5 Antibody (10T1)BSA Free [NBP1-04307]

Western Blot: IRF5 Antibody (10T1)BSA Free [NBP1-04307]

Western Blot: IRF5 Antibody (10T1) [NBP1-04307] - The extracts of Ramos, THP-1, A20 and NIH3T3 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF5 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/ Immunofluorescence: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]

Immunocytochemistry/ Immunofluorescence: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]

Immunocytochemistry/Immunofluorescence: IRF5 Antibody (10T1) [NBP1-04307] - Analysis in THP-1 cells line, stained with DAPI (Blue) for nucleus staining and antibody at 1:100 with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Immunohistochemistry-Paraffin: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]

Immunohistochemistry-Paraffin: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]

Immunohistochemistry-Paraffin: IRF5 Antibody (10T1) [NBP1-04307] - Human IRF-5 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
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Applications for IRF5 Antibody (10T1) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

2-5ug

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50

Western Blot

1:500-1:1000

Reviewed Applications

Read 1 review rated 5 using NBP1-04307 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRF5

Interferon regulatory factor 5(IRF-5) is a member of IRF family, which plays a critical role in the regulation of genes induced by viral infection, immunostimulation and cell growth regulation. The expression of IRF-5 is limited to lymphoid organs, dendritic cells, and peripheral blood lymphocytes, but is not expressed in numerous leukemia and lymphoma cell lines. Three IRFs(IRF-3, IRF-5, IRF-7) function as direct transducers of virus-mediated signaling and play a critical role in the expression of type I interferon genes and some chemokines.

Long Name

Interferon Regulatory Factor 5

Alternate Names

IBD14, interferon regulatory factor 5, IRF-5, SLEB10

Gene Symbol

IRF5

UniProt

NP_002191, NP_116032.1 (Human), Q13568 (Human)

Additional IRF5 Products

Product Documents for IRF5 Antibody (10T1) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IRF5 Antibody (10T1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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