IRF5 Antibody (10T1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-04307
![Flow (Intracellular): IRF5 Antibody (10T1) - BSA Free [NBP1-04307]](https://resources.bio-techne.com/images/products/IRF5-Antibody-10T1-Flow-Intracellular-NBP1-04307-img0010.jpg "Flow (Intracellular): IRF5 Antibody (10T1) - BSA Free [NBP1-04307]")
![Flow Cytometry: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]](https://resources.bio-techne.com/images/products/IRF5-Antibody-10T1-Flow-Cytometry-NBP1-04307-img0009.jpg "Flow Cytometry: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]")
![Western Blot: IRF5 Antibody (10T1)BSA Free [NBP1-04307]](https://resources.bio-techne.com/images/products/IRF5-Antibody-10T1-Western-Blot-NBP1-04307-img0005.jpg "Western Blot: IRF5 Antibody (10T1)BSA Free [NBP1-04307]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 10T1
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human IRF5 (176-240 aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for IRF5 Antibody (10T1) - BSA Free
Western Blot: IRF5 Antibody (10T1)BSA Free [NBP1-04307]
Western Blot: IRF5 Antibody (10T1) [NBP1-04307] - The extracts of Ramos, THP-1, A20 and NIH3T3 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF5 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.![Immunocytochemistry/ Immunofluorescence: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]](https://resources.bio-techne.com/images/products/IRF5-Antibody-10T1-Immunocytochemistry-Immunofluorescence-NBP1-04307-img0008.jpg "Immunocytochemistry/ Immunofluorescence: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]")
Immunocytochemistry/ Immunofluorescence: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]
Immunocytochemistry/Immunofluorescence: IRF5 Antibody (10T1) [NBP1-04307] - Analysis in THP-1 cells line, stained with DAPI (Blue) for nucleus staining and antibody at 1:100 with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).![Immunohistochemistry-Paraffin: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]](https://resources.bio-techne.com/images/products/IRF5-Antibody-10T1-Immunohistochemistry-Paraffin-NBP1-04307-img0003.jpg "Immunohistochemistry-Paraffin: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]")
Immunohistochemistry-Paraffin: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]
Immunohistochemistry-Paraffin: IRF5 Antibody (10T1) [NBP1-04307] - Human IRF-5 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).Applications for IRF5 Antibody (10T1) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
2-5ug
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:50
Western Blot
1:500-1:1000
Reviewed Applications
Read 1 review rated 5 using NBP1-04307 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IRF5
Long Name
Interferon Regulatory Factor 5
Alternate Names
IBD14, interferon regulatory factor 5, IRF-5, SLEB10
Gene Symbol
IRF5
UniProt
Additional IRF5 Products
Product Documents for IRF5 Antibody (10T1) - BSA Free
Product Specific Notices for IRF5 Antibody (10T1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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