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IRF5 Antibody (1H6)

Novus Biologicals, part of Bio-Techne | Catalog # H00003663-M03

Novus Biologicals, part of Bio-Techne
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H00003663-M03

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1H6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

IRF5 (NP_002191, 395 a.a. ~ 504 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TPPPFEIFFCFGEEWPDRKPREKKLITVQVVPVAARLLLEMFSGELSWSADSIRLQISNPDLKDRMVEQFKELHHIWQSQQRLQPVAQAPPGAGLGVGQGPWPMHPAGMQ

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

IRF5 - interferon regulatory factor 5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for IRF5 Antibody (1H6)

Western Blot: IRF5 Antibody (1H6) [H00003663-M03]

Western Blot: IRF5 Antibody (1H6) [H00003663-M03]

Western Blot: IRF5 Antibody (1H6) [H00003663-M03] - Analysis of IRF5 expression in NIH/3T3 (Cat # L018V1).
Immunocytochemistry/ Immunofluorescence: IRF5 Antibody (1H6) [H00003663-M03]

Immunocytochemistry/ Immunofluorescence: IRF5 Antibody (1H6) [H00003663-M03]

Immunocytochemistry/Immunofluorescence: IRF5 Antibody (1H6) [H00003663-M03] - Analysis of monoclonal antibody to IRF5 on A-431 cell. Antibody concentration 10 ug/ml
Western Blot: IRF5 Antibody (1H6) [H00003663-M03]

Western Blot: IRF5 Antibody (1H6) [H00003663-M03]

Western Blot: IRF5 Antibody (1H6) [H00003663-M03] - Analysis of IRF5 expression in A-431 (Cat # L015V1).

Applications for IRF5 Antibody (1H6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA. Use in FLOW reported in scientific literature (PMID: 30593537).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IRF5

This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist.

Long Name

Interferon Regulatory Factor 5

Alternate Names

IBD14, interferon regulatory factor 5, IRF-5, SLEB10

Entrez Gene IDs

3663 (Human)

Gene Symbol

IRF5

OMIM

607218 (Human)

UniProt

Additional IRF5 Products

Product Documents for IRF5 Antibody (1H6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IRF5 Antibody (1H6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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