Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all IRF7 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 17 amino acids near the center of human IRF7. The immunogen is located within amino acids 200-230 of IRF7.

Specificity

Several isoforms of IRF7 are known to exist. Human IRF7 has 4 isoforms, including isoform A (503aa, 54kD), isoform B (474aa, 52kD), isoform C (164aa, 18kD) and isoform D (516aa, 56kD). Mouse IRF7 has one isoform (457aa, 51kD) and Rat IRF7 also has one isoform (456aa, 51kD). NBP3-06987 can detect human isoforms and mouse, and possibly can detect rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRF7 Antibody - BSA Free

Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]

Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]

Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06987(1 ug/mL), 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution
Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]

Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]

Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 10ug of A549 WT cell lysates or IRF7 KO cell lysates. Antibodies: NBP3-06987 (0.5 ug/mL) and beta-actin, 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]

Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]

Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06987(1 ug/mL), 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution
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Applications for IRF7 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2-5 ug/mL

Immunohistochemistry-Paraffin

2-5 ug/mL

Western Blot

0.5-1 ug/mL

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRF7

IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established.

Alternate Names

interferon regulatory factor 7, interferon regulatory factor-7H, IRF-7, IRF7A, IRF-7H

Gene Symbol

IRF7

Additional IRF7 Products

Product Documents for IRF7 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IRF7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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