IRF7 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06987
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against a peptide corresponding to 17 amino acids near the center of human IRF7. The immunogen is located within amino acids 200-230 of IRF7.
Specificity
Several isoforms of IRF7 are known to exist. Human IRF7 has 4 isoforms, including isoform A (503aa, 54kD), isoform B (474aa, 52kD), isoform C (164aa, 18kD) and isoform D (516aa, 56kD). Mouse IRF7 has one isoform (457aa, 51kD) and Rat IRF7 also has one isoform (456aa, 51kD). NBP3-06987 can detect human isoforms and mouse, and possibly can detect rat.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IRF7 Antibody - BSA Free
Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]
Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06987(1 ug/mL), 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilutionImmunocytochemistry/ Immunofluorescence: IRF7 Antibody - BSA Free [NBP3-06987]
Immunocytochemistry/Immunofluorescence: IRF7 Antibody [NBP3-06987] - Immunofluorescent analysis of 4% paraformaldehyde-fixed Raw264.7 cells labeling IRF7 with NBP3-06987 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987]
Immunohistochemistry: IRF7 Antibody [NBP3-06987] - Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using NBP3-06987 at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degrees C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.Applications for IRF7 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
20 ug/ml
Immunohistochemistry
2-5 ug/mL
Immunohistochemistry-Paraffin
2-5 ug/mL
Western Blot
0.5-1 ug/mL
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IRF7
Alternate Names
interferon regulatory factor 7, interferon regulatory factor-7H, IRF-7, IRF7A, IRF-7H
Gene Symbol
IRF7
Additional IRF7 Products
Product Documents for IRF7 Antibody - BSA Free
Product Specific Notices for IRF7 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...