IRF8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1093

Novus Biologicals, part of Bio-Techne
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NB100-1093
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all IRF8 Antibodies »

Product Summary for IRF8 Antibody

Immunogen

Peptide with sequence C-QRSFFRENQQITV corresponding to C-Terminus according to NP_002154.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for IRF8 Antibody

Western Blot: IRF8 Antibody [NB100-1093]

Western Blot: IRF8 Antibody [NB100-1093]

Western Blot: IRF8 Antibody [NB100-1093] - (1ug/ml) of K562 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: IRF8 Antibody [NB100-1093]

Immunohistochemistry-Paraffin: IRF8 Antibody [NB100-1093]

Immunohistochemistry-Paraffin: IRF8 Antibody [NB100-1093] - (5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for IRF8 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

1:1000
Application Notes
WB: Approx. 45-50 kDa band observed in K562 lysate (predicted MW of 47 kDa band according to NP_002154).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IRF8

Interferon consensus sequence binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA binding domain in the N terminal region and a divergent C terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN alpha and IFN beta. IRF family proteins also control expression of IFN alpha and IFN beta regulated genes that are induced by viral infection. IRF8 specifically binds to the upstream regulatory region of type I IFN and IFN inducible MHC class I genes (the interferon consensus sequence (ICS)). Unlike IRF3, IRF8 appears to act as a negative regulator of IFN induced genes in most cases, but IRF8 mediates activation of NF kappaB by the toll like receptor 9 (TLR9) after stimulation by unmethylated CpG DNA in dendritic cells. Finally, it has been shown that IRF8 decreases bcl-2 expression and thus may play a role in chronic myelogenous leukemia

Long Name

Interferon Regulatory Factor 8

Alternate Names

ICSBP1

Entrez Gene IDs

3394 (Human); 15900 (Mouse)

Gene Symbol

IRF8

UniProt

NP_002154 (Human)

Additional IRF8 Products

Product Documents for IRF8 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IRF8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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