IRF9 Antibody (1D11)

Novus Biologicals, part of Bio-Techne | Catalog # H00010379-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IRF9 Antibodies »

Product Specifications

Immunogen

ISGF3G (AAH35716, 1 a.a. ~ 393 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASGRARCTRKLRNWVVEQVESGQFPGVCWDDTAKTMFRIPWKHAGKQDFREDQDAAFFKAWAIFKGKYKEGDTGGPAVWKTRLRCALNKSSEFKEVPERGRMDVAEPYKVYQLLPPGIVSGQPGTQKVPSKRQHSSVSSERKEEEDAMQNCTLSPSVLQDSLNNEEEGASGGAVHSDIGSSSSSSSPEPQEVTDTTEAPFQGDQRSLEFLLPPEPDYSLLLTFIYNGRVVGEAQVQSLDCRLVAEPSGSESSMEQVLFPKPGPLEPTQRLLSQLERGILVASNPRGLFVQRLCPIPISWNAPQAPPGPGPHLLPSNECVELFRTAYFCRDLVRYFQGLGPPPKFQVTLNFWEESHGSSHTPQNLITVKMEQAFARYLLEQTPEQQAAILSLV

Reactivity Notes

Human. Other species not tested.

Specificity

ISGF3G - interferon-stimulated transcription factor 3, gamma 48kDa

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for IRF9 Antibody (1D11)

Western Blot: IRF9 Antibody (1D11) [H00010379-M02]

Western Blot: IRF9 Antibody (1D11) [H00010379-M02]

Western Blot: IRF9 Antibody (1D11) [H00010379-M02] - ISGF3G monoclonal antibody (M02), clone 1D11 Analysis of ISGF3G expression in MCF-7.
Immunohistochemistry-Paraffin: IRF9 Antibody (1D11) [H00010379-M02]

Immunohistochemistry-Paraffin: IRF9 Antibody (1D11) [H00010379-M02]

Immunohistochemistry-Paraffin: IRF9 Antibody (1D11) [H00010379-M02] - Analysis of monoclonal antibody to ISGF3G on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. Antibody concentration 3 ug/ml.
Sandwich ELISA: IRF9 Antibody (1D11) [H00010379-M02] - Detection limit for recombinant GST tagged ISGF3G is approximately 0.1ng/ml as a capture antibody.

Applications for IRF9 Antibody (1D11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IRF9

Transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state

Long Name

Interferon Regulatory Factor 9

Alternate Names

ISGF3, ISGF3G

Entrez Gene IDs

10379 (Human)

Gene Symbol

IRF9

OMIM

147574 (Human)

UniProt

AAH35716 (Human)

Additional IRF9 Products

Product Documents for IRF9 Antibody (1D11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IRF9 Antibody (1D11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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