IRGM Antibody

Western Blot: IRGM Antibody [NBP3-18254]

Western Blot: IRGM Antibody [NBP3-18254] - Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of ~20.1 kDa IRGM protein using Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~20.1 kDa.

Immunocytochemistry/ Immunofluorescence: IRGM Antibody [NBP3-18254]

Immunocytochemistry/Immunofluorescence: IRGM Antibody [NBP3-18254] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.

Immunohistochemistry: IRGM Antibody [NBP3-18254]

Immunohistochemistry: IRGM Antibody [NBP3-18254] - Immunohistochemistry analysis using Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254). Tissue: Colon Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.