IRS1 Antibody (OTI3G10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45435

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3G10

Concentration

1 mg/ml

View all IRS1 Antibodies »

Product Summary for IRS1 Antibody (OTI3G10)

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-280 of human IRS1(NP_005535) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

131.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IRS1 Antibody (OTI3G10)

Western Blot: IRS1 Antibody (OTI3G10) [NBP2-45435]

Western Blot: IRS1 Antibody (OTI3G10) [NBP2-45435]

Western Blot: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRS1.
Immunohistochemistry: IRS1 Antibody (OTI3G10) [NBP2-45435]

Immunohistochemistry: IRS1 Antibody (OTI3G10) [NBP2-45435]

Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: IRS1 Antibody (OTI3G10) [NBP2-45435]

Immunohistochemistry: IRS1 Antibody (OTI3G10) [NBP2-45435]

Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
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Applications for IRS1 Antibody (OTI3G10)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IRS1

Insulin receptor substrates (IRS), the major intracellular substrates of the insulin receptor (IR), are adaptor proteins that transduce signals from the IR to downstream effectors that are important for the biological effect of insulin (1-2). After insulin stimulation, IRS proteins are rapidly phosphorylated on multiple tyrosine residues. Once phosphorylated, IRS proteins bind and activate Grb-2, SHP2 and the PI3-K p85 subunit (2-3). IRS-1 functions as one of the key regulators of IR and the insulin-like growth factor-1 receptor. Disruption of IRS-1 causes growth retardation and insulin resistance associated with hypertension, hypertriglyceridemia, and impaired endothelium-dependent vascular relaxation (4-5)

Long Name

Insulin Receptor Substrate 1

Alternate Names

HIRS-1, insulin receptor substrate 1, IRS-1

Gene Symbol

IRS1

Additional IRS1 Products

Product Documents for IRS1 Antibody (OTI3G10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IRS1 Antibody (OTI3G10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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