Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all IRS2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1288 and 1338 of human insulin receptor substrate 2 using the numbering given in entry NP_003740.2 (GeneID 8660).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRS2 Antibody

Immunohistochemistry-Paraffin: IRS2 Antibody [NB100-68244]

Immunohistochemistry-Paraffin: IRS2 Antibody [NB100-68244]

Immunohistochemistry-Paraffin: IRS2 Antibody [NB100-68244] - Human ovarian carcinoma. Antibody used at a dilution of 1:1,000.
Immunoprecipitation: IRS2 Antibody [NB100-68244]

Immunoprecipitation: IRS2 Antibody [NB100-68244]

Immunoprecipitation: IRS2 Antibody [NB100-68244] - Detection of Human IRS2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-IRS2 antibody NB100-68244 used for IP at 3 mcg/mg lysate. IRS2 was also immunoprecipitated by rabbit anti-IRS2 antibodies NB100-68242 and NB100-68243, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).

Applications for IRS2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2 - 5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IRS2

This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment.

Long Name

Insulin Receptor Substrate 2

Alternate Names

insulin receptor substrate 2, IRS-2

Entrez Gene IDs

8660 (Human)

Gene Symbol

IRS2

UniProt

NP_003740.2 (Human)

Additional IRS2 Products

Product Documents for IRS2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IRS2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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