IRX1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83090

Novus Biologicals, part of Bio-Techne
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NBP1-83090
NBP1-83090-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IRX1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NKVTWGARSKDQEDGALFGSDTEGDPEKAEDDEEIDLESIDIDKIDEHDGDQSNEDDEDKAEAPHAPAAPSALARDQGSPLAAADVLKPQDS

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34155146). Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRX1 Antibody

Western Blot: IRX1 Antibody [NBP1-83090]

Western Blot: IRX1 Antibody [NBP1-83090]

Western Blot: IRX1 Antibody [NBP1-83090] - Analysis in human heart tissue.
Immunocytochemistry/ Immunofluorescence: IRX1 Antibody [NBP1-83090]

Immunocytochemistry/ Immunofluorescence: IRX1 Antibody [NBP1-83090]

Immunocytochemistry/Immunofluorescence: IRX1 Antibody [NBP1-83090] - Staining of human cell line REH shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: IRX1 Antibody [NBP1-83090]

Immunohistochemistry-Paraffin: IRX1 Antibody [NBP1-83090]

Immunohistochemistry-Paraffin: IRX1 Antibody [NBP1-83090] - Staining of mouse embryo E14 shows moderate to strong nuclear positivity in developing teeth.
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Applications for IRX1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 -1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRX1

The Iroquois homeobox gene family of transcription factors regulate aspects of embryonic development including anterior/posterior and dorsal/ventral axis patterning in the central nervous system. The Iroquois family are clustered on two loci, IRXA and IRXB, which map to chromosomes 8 and 13 in mice. The IRXA group includes Irx1, Irx2 and Irx4; the IRXB group is comprises Irx3, Irx5 and Irx6. Irx1 and Irx2 are both widely expressed during development in the lung epithelium and also in the ventricular septum. Irx1 and Irx2 also play a role in digit formation (E11.5-E14.5). The Irx gene family members are each expressed in a distinct pattern during mouse heart development. Specifically, Irx1 and Irx2 are expressed in the ventricular septum and Irx3 is expressed in the ventricular trabeculated myocardium. In addition, Irx4 is expressed in the linear heart tube and the AV canal; Irx5 is expressed in the endocardium lining the ventricular and atrial myocardium. Furthermore, the IRX4 gene may modulate cardiac development and function. Although the heart of Irx4- mice appears to develop normally, adult Irx4- mice exhibit cardiomyopathy, including cardiac hypertrophy and decreased contractility.

Alternate Names

Homeodomain protein IRXA1, iroquois homeobox 1, Iroquois homeobox protein 1, iroquois-class homeodomain protein IRX-1, IRX-5, IRXA1

Gene Symbol

IRX1

Additional IRX1 Products

Product Documents for IRX1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IRX1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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