IRX6 Antibody (1A5)
Novus Biologicals, part of Bio-Techne | Catalog # H00079190-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1A5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
IRX6 (NP_077311, 337 a.a. ~ 446 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PRLTMHYPCLEKPRIWSLAHTATASAVEGAPPARPRPRSPECRMIPGQPPASARRLSVPRDSACDESSCIPKAFGNPKFALQGLPLNCAPCPRRSEPVVQCQYPSGAEAG
Reactivity Notes
Human. Other species not tested.
Specificity
IRX6 (1A5)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for IRX6 Antibody (1A5)
![Western Blot: IRX6 Antibody (1A5) [H00079190-M01]](https://resources.bio-techne.com/images/products/IRX6-Antibody-1A5-Western-Blot-H00079190-M01-img0005.jpg "Western Blot: IRX6 Antibody (1A5) [H00079190-M01]")
Western Blot: IRX6 Antibody (1A5) [H00079190-M01]
Western Blot: IRX6 Antibody (1A5) [H00079190-M01] - Analysis of IRX6 expression in transfected 293T cell line by IRX6 monoclonal antibody (M01), clone 1A5.Lane 1: IRX6 transfected lysate(48.169 KDa).Lane 2: Non-transfected lysate.![Western Blot: IRX6 Antibody (1A5) [H00079190-M01]](https://resources.bio-techne.com/images/products/IRX6-Antibody-1A5-Western-Blot-H00079190-M01-img0003.jpg "Western Blot: IRX6 Antibody (1A5) [H00079190-M01]")
Western Blot: IRX6 Antibody (1A5) [H00079190-M01]
Western Blot: IRX6 Antibody (1A5) [H00079190-M01] - Analysis of IRX6 over-expressed 293 cell line, cotransfected with IRX6 Validated Chimera RNAi ( Cat # H00079190-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IRX6 monoclonal antibody (M01), clone 1A5 (Cat # H00079190-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: IRX6 Antibody (1A5) [H00079190-M01] - Detection limit for recombinant GST tagged IRX6 is 0.1 ng/ml as a capture antibody.
Applications for IRX6 Antibody (1A5)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for Western Blot. It has also been used for ELISA and RNAi validation.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: IRX6
Alternate Names
iroquois homeobox 6, Iroquois homeobox protein 6Homeodomain protein IRXB3, iroquois homeobox protein 7, iroquois-class homeodomain protein IRX-6, IRX-3, IRX7IRXB3
Entrez Gene IDs
79190 (Human)
Gene Symbol
IRX6
OMIM
606196 (Human)
UniProt
Additional IRX6 Products
Product Documents for IRX6 Antibody (1A5)
Product Specific Notices for IRX6 Antibody (1A5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...