ISG15/UCRP Antibody (2C6V1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16129
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2C6V1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for ISG15/UCRP Antibody (2C6V1)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human ISG15 (NP_005092.1). MGWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRLAVHPSGVALQDRVPLASQGLGPGSTVLLVVDKCDEPLSILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPLSTVFMNLRLRGGGTEPGGRS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ISG15/UCRP Antibody (2C6V1)
Western Blot: ISG15/UCRP Antibody (ARC53794) [NBP3-16129] -
Western Blot: ISG15/UCRP Antibody (ARC53794) [NBP3-16129] - Analysis of extracts from wild type(WT) and ISG15 Rabbit mAb knockdown (KD) Hela(KD) cells, using ISG15 Rabbit mAb antibody at 1:510 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.
ISG15/UCRP Antibody (ARC53794) [NBP3-16129] -
ISG15/UCRP Antibody (ARC53794) [NBP3-16129] -Confocal imaging of U-2 OS cells using [KD Validated] ISG15 Rabbit mAb (dilution 1:100) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.Applications for ISG15/UCRP Antibody (2C6V1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ISG15/UCRP
Long Name
ISG15 Ubiquitin-like Modifier
Alternate Names
G1P2, IFI15, IP17, UCRP
Gene Symbol
ISG15
Additional ISG15/UCRP Products
Product Documents for ISG15/UCRP Antibody (2C6V1)
Product Specific Notices for ISG15/UCRP Antibody (2C6V1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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